Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan
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چکیده
منابع مشابه
Protocorms and Protocorm-Like Bodies Are Molecularly Distinct from Zygotic Embryonic Tissues in Phalaenopsis aphrodite.
The distinct reproductive program of orchids provides a unique evolutionary model with pollination-triggered ovule development and megasporogenesis, a modified embryogenesis program resulting in seeds with immature embryos, and mycorrhiza-induced seed germination. However, the molecular mechanisms that have evolved to establish these unparalleled developmental programs are largely unclear. Here...
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Carbon and nitrogen are the two elements that most affect plant organogenesis. In vitro, usually as part of the macronutrients. Some anecdotal evidence from the literature suggests that the ammonium (NH4+) to nitrate (NO3–) ratio may affect orchid organogenesis. In this study, to test this hypothesis, different NH4+: NO3– ratios were tested on the development of protocorm-like bodies (PLBs) of ...
متن کاملPhysical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids.
Phalaenopsis orchids have been regenerated by inducing protocorm-like bodies (PLBs) from etiolated leaf sections. However, the physiological and molecular mechanisms of secondary PLB development and subsequent proliferation have not been explored. Bisectionally cutting primary PLBs resulted in more secondary PLBs at 5 weeks, suggesting an embryogenic stem cell property imposed by wounding of pr...
متن کاملammonium to nitrate ratio affects protocorm like bodies plb formation in vitro of hybrid cymbidium
carbon and nitrogen are the two elements that most affect plant organogenesis. in vitro, usually as part of the macronutrients. some anecdotal evidence from the literature suggests that the ammonium (nh4+) to nitrate (no3–) ratio may affect orchid organogenesis. in this study, to test this hypothesis, different nh4+: no3– ratios were tested on the development of protocorm-like bodies (plbs) of ...
متن کاملThidiazuron Triggers Morphogenesis in Rosa canina L. Protocorm-Like Bodies by Changing Incipient Cell Fate
Thidiazuron (N-phenyl-N'-1,2,3-thiadiazol-5-ylurea; TDZ) is an artificial plant growth regulator that is widely used in plant tissue culture. Protocorm-like bodies (PLBs) induced by TDZ serve as an efficient and rapid in vitro regeneration system in Rosa species. Despite this, the mechanism of PLB induction remains relatively unclear. TDZ, which can affect the level of endogenous auxins and cyt...
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ژورنال
عنوان ژورنال: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
سال: 2013
ISSN: 1842-4309,0255-965X
DOI: 10.15835/nbha4118315